Futterman From United States of America, joined Sep 2003, 1301 posts, RR: 49 Posted (9 years 1 week 6 days 19 hours ago) and read 850 times:
Yet another bump in the road I have come to know as 'Science Project.' To the chase, can standard curves be used for comparative purposes?
I understand how they can be used to determine, for instance, the size of an unidentified protein, but can I put two curves (two separate data series) on the same graph, and use it to merely compare things?
Perhaps, since I have six graphs, simply comparing the curves or plots between the graphs is one way. Did I just answer my own question?
I'm trying to get a photo up so maybe you can get the jist of what I'm trying to ask.
Vafi88 From United States of America, joined Apr 2001, 3116 posts, RR: 19 Reply 1, posted (9 years 1 week 6 days 19 hours ago) and read 830 times:
Brian, I thinik you need to explain to these folks exactly what you're doing, I don't think anyone understood you.
ME knowing what you're doing, I'd have to say you have to compare the 2 (remember the chicken/shrimp ordeal) graphs possibly on one graph.
If you get a standard, what would you get a standard of? Like you said the tissues are different as you go from species to species such as beef and pork might be the same but chicken and shrimp might be totally different.
I'd like to elect a president that has a Higher IQ than a retarted ant.
Futterman From United States of America, joined Sep 2003, 1301 posts, RR: 49 Reply 4, posted (9 years 1 week 6 days 18 hours ago) and read 801 times:
"Using Protein Electrophoresis to Determine Protein Similarities Among Different Organisms" is the title of my experiment. Here's a shot of one of the graphs (Beef...there's also Chicken, Pork, Octopus, Chicken, and Fish, plus kaleidoscope and actin/myosin standards).
Vitaly: The standard is something called a Kaleidoscope Standard (refer to link for definition). In electrophoresis, you run that as well as the samples (each in separate lanes). All of the banding patterns (refer to link again) that appear from the Kaleidoscope Standard have 'pre-determined', or known molecular weights. This is another way to identify unknown proteins in my project. http://www.bio-rad.com/LifeScience/pdf/Bulletin_2123.pdf