Well, it depends.
If your graphics card has a built in thermocouple (as many of them do) then yes, it is possible to measure the temperature using software, just as you can measure the temp of your CPU, Motherboard etc - do a search on the net to find out if your card has the hardware required and software available to do this.
The ATI Radeon in one of my work PC
's has a built in thermocouple, and I got a utility program along with it to query it's temperature. I can also query it by probing the hardware directly through some code.
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