Depending what camera and what lens i am using depends what settings i use.
For every shot i try and brace myself against a wall/seat etc to steady my body, i slowly breathe out as i trip the shutter and i always try to shoot in a burst of 3/4 shots in quick succesion to increase chance of a good one.
On my 10D i never go above ISO 400, occasionally 800 but that's a rare occurance. On my 1dmkii with the extra crop i can drop the shutter speed a little more and also push the ISO to 800, sometimes 1600. When shooting at high ISO you must try not to over/under expose. Always use the histogram to try and balance the exposure well as at higher iso's grain is an issue when trying to pull back an under exposed shot in photoshop.
I hope i make some sense!
5D2/7D/1D2(soon to be a 1Dx) 17-40L/24-105L/70-200F2.8L/100-400L/24F1.4LII/50F1.2L/85F1.2LII